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A A schematic setup is shown for stimulation with the Tie2 agonist PMC-403 and its effects on HUVECs and HPMECs, accordingly. Created in BioRender. Stahl, K. (2026) https://BioRender.com/yc7s3fj . B HUVECs were stimulated with different concentrations of the Tie2 agonist PMC-403 or 500 <t>ng/ml</t> <t>Angpt-1</t> for 1 hour. A western blot was performed and pAKT and AKT were analyzed. C HPMECs were incubated with 1 µg/ml PMC-403 for 1 hour and then stimulated with LPS. TER was measured by ECIS over time and group differences over time were analyzed by a linear mixed-effects model accordingly. D Group differences are shown 6.5 hours after LPS stimulation. Results are shown as dot plots with mean ± SD and each group consisted of n =4. P -values < 0.05 are considered significant. Abbreviations: Angpt-1 - Angiopoietin-1, ECIS - Electric cell-substrate impedance sensing, HPMECs - human pulmonary microvascular endothelial cells, HUVECs – human umbilical vein endothelial cells, LPS - lipopolysaccharide, SD – standard deviation, TER – transendothelial electrical resistance
Angpt 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A A schematic setup is shown for stimulation with the Tie2 agonist PMC-403 and its effects on HUVECs and HPMECs, accordingly. Created in BioRender. Stahl, K. (2026) https://BioRender.com/yc7s3fj . B HUVECs were stimulated with different concentrations of the Tie2 agonist PMC-403 or 500 <t>ng/ml</t> <t>Angpt-1</t> for 1 hour. A western blot was performed and pAKT and AKT were analyzed. C HPMECs were incubated with 1 µg/ml PMC-403 for 1 hour and then stimulated with LPS. TER was measured by ECIS over time and group differences over time were analyzed by a linear mixed-effects model accordingly. D Group differences are shown 6.5 hours after LPS stimulation. Results are shown as dot plots with mean ± SD and each group consisted of n =4. P -values < 0.05 are considered significant. Abbreviations: Angpt-1 - Angiopoietin-1, ECIS - Electric cell-substrate impedance sensing, HPMECs - human pulmonary microvascular endothelial cells, HUVECs – human umbilical vein endothelial cells, LPS - lipopolysaccharide, SD – standard deviation, TER – transendothelial electrical resistance
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hy p1455a angiopoietin 1 angpt 1 endogenous - by Bioz Stars, 2026-10
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A A schematic setup is shown for stimulation with the Tie2 agonist PMC-403 and its effects on HUVECs and HPMECs, accordingly. Created in BioRender. Stahl, K. (2026) https://BioRender.com/yc7s3fj . B HUVECs were stimulated with different concentrations of the Tie2 agonist PMC-403 or 500 <t>ng/ml</t> <t>Angpt-1</t> for 1 hour. A western blot was performed and pAKT and AKT were analyzed. C HPMECs were incubated with 1 µg/ml PMC-403 for 1 hour and then stimulated with LPS. TER was measured by ECIS over time and group differences over time were analyzed by a linear mixed-effects model accordingly. D Group differences are shown 6.5 hours after LPS stimulation. Results are shown as dot plots with mean ± SD and each group consisted of n =4. P -values < 0.05 are considered significant. Abbreviations: Angpt-1 - Angiopoietin-1, ECIS - Electric cell-substrate impedance sensing, HPMECs - human pulmonary microvascular endothelial cells, HUVECs – human umbilical vein endothelial cells, LPS - lipopolysaccharide, SD – standard deviation, TER – transendothelial electrical resistance
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A A schematic setup is shown for stimulation with the Tie2 agonist PMC-403 and its effects on HUVECs and HPMECs, accordingly. Created in BioRender. Stahl, K. (2026) https://BioRender.com/yc7s3fj . B HUVECs were stimulated with different concentrations of the Tie2 agonist PMC-403 or 500 <t>ng/ml</t> <t>Angpt-1</t> for 1 hour. A western blot was performed and pAKT and AKT were analyzed. C HPMECs were incubated with 1 µg/ml PMC-403 for 1 hour and then stimulated with LPS. TER was measured by ECIS over time and group differences over time were analyzed by a linear mixed-effects model accordingly. D Group differences are shown 6.5 hours after LPS stimulation. Results are shown as dot plots with mean ± SD and each group consisted of n =4. P -values < 0.05 are considered significant. Abbreviations: Angpt-1 - Angiopoietin-1, ECIS - Electric cell-substrate impedance sensing, HPMECs - human pulmonary microvascular endothelial cells, HUVECs – human umbilical vein endothelial cells, LPS - lipopolysaccharide, SD – standard deviation, TER – transendothelial electrical resistance
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A A schematic setup is shown for stimulation with the Tie2 agonist PMC-403 and its effects on HUVECs and HPMECs, accordingly. Created in BioRender. Stahl, K. (2026) https://BioRender.com/yc7s3fj . B HUVECs were stimulated with different concentrations of the Tie2 agonist PMC-403 or 500 <t>ng/ml</t> <t>Angpt-1</t> for 1 hour. A western blot was performed and pAKT and AKT were analyzed. C HPMECs were incubated with 1 µg/ml PMC-403 for 1 hour and then stimulated with LPS. TER was measured by ECIS over time and group differences over time were analyzed by a linear mixed-effects model accordingly. D Group differences are shown 6.5 hours after LPS stimulation. Results are shown as dot plots with mean ± SD and each group consisted of n =4. P -values < 0.05 are considered significant. Abbreviations: Angpt-1 - Angiopoietin-1, ECIS - Electric cell-substrate impedance sensing, HPMECs - human pulmonary microvascular endothelial cells, HUVECs – human umbilical vein endothelial cells, LPS - lipopolysaccharide, SD – standard deviation, TER – transendothelial electrical resistance
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A A schematic setup is shown for stimulation with the Tie2 agonist PMC-403 and its effects on HUVECs and HPMECs, accordingly. Created in BioRender. Stahl, K. (2026) https://BioRender.com/yc7s3fj . B HUVECs were stimulated with different concentrations of the Tie2 agonist PMC-403 or 500 <t>ng/ml</t> <t>Angpt-1</t> for 1 hour. A western blot was performed and pAKT and AKT were analyzed. C HPMECs were incubated with 1 µg/ml PMC-403 for 1 hour and then stimulated with LPS. TER was measured by ECIS over time and group differences over time were analyzed by a linear mixed-effects model accordingly. D Group differences are shown 6.5 hours after LPS stimulation. Results are shown as dot plots with mean ± SD and each group consisted of n =4. P -values < 0.05 are considered significant. Abbreviations: Angpt-1 - Angiopoietin-1, ECIS - Electric cell-substrate impedance sensing, HPMECs - human pulmonary microvascular endothelial cells, HUVECs – human umbilical vein endothelial cells, LPS - lipopolysaccharide, SD – standard deviation, TER – transendothelial electrical resistance
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A A schematic setup is shown for stimulation with the Tie2 agonist PMC-403 and its effects on HUVECs and HPMECs, accordingly. Created in BioRender. Stahl, K. (2026) https://BioRender.com/yc7s3fj . B HUVECs were stimulated with different concentrations of the Tie2 agonist PMC-403 or 500 ng/ml Angpt-1 for 1 hour. A western blot was performed and pAKT and AKT were analyzed. C HPMECs were incubated with 1 µg/ml PMC-403 for 1 hour and then stimulated with LPS. TER was measured by ECIS over time and group differences over time were analyzed by a linear mixed-effects model accordingly. D Group differences are shown 6.5 hours after LPS stimulation. Results are shown as dot plots with mean ± SD and each group consisted of n =4. P -values < 0.05 are considered significant. Abbreviations: Angpt-1 - Angiopoietin-1, ECIS - Electric cell-substrate impedance sensing, HPMECs - human pulmonary microvascular endothelial cells, HUVECs – human umbilical vein endothelial cells, LPS - lipopolysaccharide, SD – standard deviation, TER – transendothelial electrical resistance

Journal: Respiratory Research

Article Title: The Tie-2 agonist PMC-403 inhibits endothelial damage induced by e-cigarette vapor or LPS

doi: 10.1186/s12931-026-03928-4

Figure Lengend Snippet: A A schematic setup is shown for stimulation with the Tie2 agonist PMC-403 and its effects on HUVECs and HPMECs, accordingly. Created in BioRender. Stahl, K. (2026) https://BioRender.com/yc7s3fj . B HUVECs were stimulated with different concentrations of the Tie2 agonist PMC-403 or 500 ng/ml Angpt-1 for 1 hour. A western blot was performed and pAKT and AKT were analyzed. C HPMECs were incubated with 1 µg/ml PMC-403 for 1 hour and then stimulated with LPS. TER was measured by ECIS over time and group differences over time were analyzed by a linear mixed-effects model accordingly. D Group differences are shown 6.5 hours after LPS stimulation. Results are shown as dot plots with mean ± SD and each group consisted of n =4. P -values < 0.05 are considered significant. Abbreviations: Angpt-1 - Angiopoietin-1, ECIS - Electric cell-substrate impedance sensing, HPMECs - human pulmonary microvascular endothelial cells, HUVECs – human umbilical vein endothelial cells, LPS - lipopolysaccharide, SD – standard deviation, TER – transendothelial electrical resistance

Article Snippet: Unless specified otherwise, cells were stimulated with 100 ng/ml lipopolysaccharide (LPS) from E. coli serotype O111:B4 (L4130, Sigma-Aldrich, St. Louis, MO) or vehicle (EBM-2), 100 ng/ml lipopolysaccharide binding protein (LBP) (870-LP-025, R&D Systems, Minneapolis, MN), 100 ng/ml CD14 (SRP3149, Sigma–Aldrich, St. Louis, MO), 500 ng/ml Angpt-1 (923-AN/CF, R&D Systems, Minneapolis, MN), electronic cigarette vapor extract (ECVE, see below) and/or different concentrations (e.g. 0.3, 1, 3–10 μg/ml) of the Tie2 agonist PMC-403 (PharmAbcine, Daejeon, Republic of Korea).

Techniques: Western Blot, Incubation, Electric Cell-substrate Impedance Sensing, Standard Deviation